<h1 id="2023-05-26">2023-05-26<a aria-hidden="true" class="anchor-heading icon-link" href="#2023-05-26"></a></h1>
<h1 id="this-is-audreys-dbgi-daily-open-notebook">This is Audrey's DBGI daily open-notebook.<a aria-hidden="true" class="anchor-heading icon-link" href="#this-is-audreys-dbgi-daily-open-notebook"></a></h1>
<p>Today is 2023.05.26</p>
<h2 id="todo-today">Todo today<a aria-hidden="true" class="anchor-heading icon-link" href="#todo-today"></a></h2>
<h3 id="have-a-look-at-the-dbgi-discussion-forum">Have a look at the DBGI discussion forum<a aria-hidden="true" class="anchor-heading icon-link" href="#have-a-look-at-the-dbgi-discussion-forum"></a></h3>
<pre><code>- https://github.com/orgs/digital-botanical-gardens-initiative/discussions
</code></pre>
<h3 id=""><a aria-hidden="true" class="anchor-heading icon-link" href="#"></a></h3>
<h3 id="-1"><a aria-hidden="true" class="anchor-heading icon-link" href="#-1"></a></h3>
<h2 id="doing">Doing<a aria-hidden="true" class="anchor-heading icon-link" href="#doing"></a></h2>
<h2 id="quality-control-for-ms-analysis">Quality control for MS analysis<a aria-hidden="true" class="anchor-heading icon-link" href="#quality-control-for-ms-analysis"></a></h2>
<p>To monitor changes in analyses over time, we would like to set up a control system. 5 plantes were selected (dbgi_001028; dbgi_001029; dbgi_001030; dbgi_001031; dbgi_001032)</p>
<h3 id="protocol-">Protocol :<a aria-hidden="true" class="anchor-heading icon-link" href="#protocol-"></a></h3>
<ul>
<li>5 g of grinded plant (with a motar) is extracted in 250 mL of methanol 100% using ultrasound bath during 30 minutes. </li>
<li>The mixture is filtered on paper filter  </li>
<li>The supernatant is transfered in tared balloon and evorapoted to dryness using a rotary evaporator </li>
<li>The dry extract is then solubilized at 5 mg/mL using methanol and aliquotate and conserved in the freezer. 100 vials of 800 µL are realised per plant. </li>
</ul>
<h2 id="paused">Paused<a aria-hidden="true" class="anchor-heading icon-link" href="#paused"></a></h2>
<h2 id="done">Done<a aria-hidden="true" class="anchor-heading icon-link" href="#done"></a></h2>
<h2 id="notes">Notes<a aria-hidden="true" class="anchor-heading icon-link" href="#notes"></a></h2>
<h2 id="todo-tomorrow">Todo tomorrow<a aria-hidden="true" class="anchor-heading icon-link" href="#todo-tomorrow"></a></h2>
<h3 id="-2"><a aria-hidden="true" class="anchor-heading icon-link" href="#-2"></a></h3>
<h3 id="-3"><a aria-hidden="true" class="anchor-heading icon-link" href="#-3"></a></h3>
<h3 id="-4"><a aria-hidden="true" class="anchor-heading icon-link" href="#-4"></a></h3>
<h2 id="today-i-learned-that">Today I learned that<a aria-hidden="true" class="anchor-heading icon-link" href="#today-i-learned-that"></a></h2>
<ul>
<li></li>
</ul>

This is Audrey's DBGI daily open-notebook.

2023-05-26


# Overview

The Digital Botanical Gardens Initiative (DBGI, www.dbgi.org) ambitions to explore innovative solutions for the collection, management and sharing of digital information acquired on living botanical collections. A particular focus will be placed on the large scale characterization of the chemodiversity of living plants collections through mass spectrometric approaches. The acquired data will be structured, organized and connected with relevant metadata through [semantic web](https://en.wikipedia.org/wiki/Semantic_Web) technology. The gathered knowledge will then inform ecosystem functioning research and orient [biodiversity conservation](https://en.wikipedia.org/wiki/Conservation_biology) projects. The DBGI initially aims to take advantage of the readily available living collections of Swiss botanical gardens to establish robust and scalable biodiversity digitisation workflows. The ultimate goal is to apply these approaches in the field and at the global scale in wildlife ecosystems. 

# Main goals

The DBGI main goals are briefly summarized below :

![](/assets/images/2022-06-18-17-13-23.png){max-width: 400px, display: block, margin: 0 auto}

# An Open Science project


## Working with the workshop doors open

![](/assets/images/2022-06-18-17-47-56.png){max-width: 400px}

A way of working briefly described by Andy Matuschak here https://notes.andymatuschak.org/z21cgR9K3UcQ5a7yPsj2RUim3oM2TzdBByZu. 


## Open Notebook Science

If we apply this "working with the workshop doors open" idea to a scientific research endeavor we understand it as respecting the core concepts of Open Science. These is more precisely the principle of Open Notebook Science https://en.wikipedia.org/wiki/Open-notebook_science

For the projects of the DBGI we will follow such an Open Notebook Science approach in order to share early ideas and results but also the less shiny parts of the research projects. 

You can follow the DBGI related Open Notebooks here [[open-notebook]].

Expect to find strange ideas, badly formulated text and poorly written code. However if you have time and interest you should be able to participate at each step of the process. Hopefully this will lead to enhanced collaboration and participation. Maybe yes, maybe not. Let's make it a methodological experiment !

Each pages of this website are written in markdown and hosted on Github. You can edit them by clicking on the "Click here to edit this page on Github !" link at the bottom of the page. See down there 👇

If you are willing to participate the the DBGI Dendron notes have a look at the [[welcome]] section to get you started !


## What's new ?!

This web site might not necessarily seem to be change since the last time you came to visit.

If something moved recently you should be able to track this over there https://github.com/digital-botanical-gardens-initiative/dendron-dbgi/commits/main